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Born 15/7/1984, in Minsk, Belarus
Education:
2014 Ph.D. degree in Biophysics at the Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic; thesis advisor: J. Lazar, Ph.D. Thesis title: “Studies of membrane protein structure and function using polarization microscopy”. Graduated with honors.
2006 Master’s degree in Biochemistry at the Belarusian State University in Minsk, Belarus; thesis advisor: M. Sholukh, Ph.D. Master’s thesis title: “Studies of the cytoprotective properties of prostaglandin groups A and E”. Graduated with honors.
2005 Bachelor’s degree in Biology at the Belarusian State University in Minsk, Belarus.
Employment History:
2023-present
2020-2022
2008-2022
2019-2022
2017-2019
2015-2017
Scientific officer (joint appointment) Laboratory of Microscopy and Histology, Institute of Entomology, Biology Centre CAS & Department of Molecular Biology and Genetics, Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic – microscopy facility management, maintenance, expert assistance to users
Research fellow (part time), Faculty of Science, University of South Bohemia in Ceske Budejovice, Czech Republic – studies of G protein signaling mechanisms in live cells
Research fellow, Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology, Academy of Sciences, Czech Republic – development of single-molecule microscopy techniques and their application to cell signaling
Research fellow (part time), Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Czech Republic – development of sensors for polarization microscopy
Postdoctoral fellow, Augusta University, Augusta, GA, USA – single-molecule studies of G protein signaling dynamics
Research fellow (part time), Institute of Organic Chemistry and Biochemistry, Academy of Sciences, Czech Republic – development of microscopy techniques
2014 (6 months)
2013 (6 months)
Postdoctoral fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with lipid membrane components
Visiting Research Fellow, Tampere University of Technology, Finland – computational studies of G protein interactions with G protein-coupled receptors
2008-2013
Research Assistant (part time), Faculty of Science, University of South Bohemia, Czech Republic – development of imaging techniques for studies of membrane proteins
2006-2007
Research Assistant (part time), Belarusian State University, Belarus – biochemical studies of antioxidant properties of synthetic prostaglandin analogs
Awards and Fellowships (selected):
2022 US and EU Patent application 18/057,629 (co-inventor with J. Lazar): Probe and method for detecting membrane-associated proteins in living cells
2020-2023 INTER-COST grant “Studies of GPCR and G-protein localization in membrane domains using single-molecule imaging”
2020-2022Junior GAČR grant “Role of receptor/G protein preassembly in G protein signaling”
2017-2019Junior GAČR grant “Insights into mechanisms of G protein signaling by the β2-adrenergic receptor”
2016Keystone “Future of Science” scholarship to attend the conference “G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery”, Keystone CO, USA.
2015 Award of the Czechoslovak Microscopy society for the best dissertation involving utilization of microscopy techniques
2014 Award of the University of South Bohemia for outstanding scientific contribution in dissertation
2014 Keystone “Future of Science” scholarship to attend the conference “G protein-coupled receptors: structural dynamics and functional implications”, Snowbird UT, USA.
2012 FEBS Youth Travel Fund grant to attend the workshop “Dynamics of cell signal systems workshop”, Oslo, Norway
2010 FEBS Youth Travel Fund grant to attend the workshop “Spatial and temporal regulation of signaling”, Oslo, Norway
2009 EMBO travel fellowship to attend the course “Advanced optical microscopy”, UK.
2009 FEBS Youth Travel Fund grant to attend the course “Practical course in computational biology”, Split, Croatia.
2008 FEBS Youth Travel Fund grant to attend the course “Microspectroscopy: monitoring molecular interactions in living cells”, Wageningen, Holland.
2008 Student research grant of the Grant Agency of the University of South Bohemia
Publications (Total number of citations (WOS): 197; H-index 7):
Petelak A., Lambert N.A., Bondar A. 5-HT7 receptor slows down the Gs protein: a single molecule perspective Molecular Biology of the Cell (2023) [IF 3.3, cited 0x]
Bondar A., Rybakova O., Melcr J., Dohnalek J., Khoroshyy P., Tichacek O., Timr S., Miclea P., Sakhi A., Markova V., Lazar J. Quantitative Linear Dichroism Imaging of Molecular Processes in Living Cells Made Simple by Open Software Tools. Communications Biology (2021) [IF 6.3, cited 1x]
Bondar A.*, Lazar J. Optical sensors of heterotrimeric G protein signaling. FEBS Journal (2021) [IF 5.5, cited 5x] (*corresponding author)
Myskova J., Rybakova O., Brynda J., Khoroshyy P., Bondar A., Lazar J. Directionality of light absorption and emission in representative fluorescent proteins. PNAS (2020) [IF 11.2, cited 15x]
Bondar A.*, Jang W., Sviridova E., Lambert N.A. Components of the Gssignaling cascade exhibit distinct changes in mobility and membrane domain localization upon β2‐adrenergic receptor activation. Traffic (2020) [IF 6.2, cited 4x] (*co-corresponding author)
Bondar A.*, Lazar J. The G protein Gi1 exhibits basal coupling but not preassembly with G protein-coupled receptors. Journal of Biological Chemistry (2017) [IF 4.0, cited 9x] (*corresponding author)
Sviridova E., Rezacova P., Bondar A., Veverka V., Novak P., Schenk G., Svergun D., Kuta Smatanova I., Bumba L. Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis. Scientific Reports (2017). [IF 4.1, cited 6x]
Timr S., Brabec J., Bondar A., Ryba T., Zelezny M., Lazar J., Jungwirth P. Nonlinear Optical Properties of Fluorescent Dyes Allow for Accurate Determination of Their Molecular Orientations in Phospholipid Membranes. Journal of Physical Chemistry B (2015). [IF 3.3, cited 10x]
Han Z., Jin L., Chen F., Loturco J.J., Cohen L.B., Bondar A., Lazar J., Pieribone V.A. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLoS ONE (2014). [IF 3.5, cited 38x]
Bondar A.*, Lazar J. Dissociated GaGTP and Gbg protein subunits are the major activated form of heterotrimeric Gi/o proteins. Journal of Biological Chemistry (2014). [IF 4.6, cited 17x] (*corresponding author)
Timr S., Bondar A., Cwiklik L., Stefl M., Hof M., Vazdar M., Lazar J. and Jungwirth P. Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane. Journal of Physical Chemistry B (2014) [IF 3.4, cited 22x]
Lazar J., Bondar A., Timr S., Firestein S., Two-photon polarization microscopy reveals protein structure and function. Nature Methods (2011). [IF 20.7, cited 65x]
Hubich , Bondar A., Kastsiuk T., Kastsiuk U., Lakhvich F., Sholukh M., Hepatoprotective action of prostaglandin A2 analogs under CCl4-induced liver injury in vitro. Hepatology Research (2007). [IF 1.892, cited 6x]
Conference Presentations (selected):
2020 Bondar A. Preassembly between the G protein-coupled receptors and G proteins studied at the single-molecule level // 3rd COST ERNEST meeting (Invited talk)
2018 Bondar A., Lambert N.A. G proteins avoid, but are not excluded from, clathrin-coated structures // Gordon Research Conference Phosphorylation and G-Protein Mediated Signaling Networks, Biddeford, ME, USA (Oral presentation)
2016 Bondar A., Brzakova A., Timr S., Lazar J. Precoupling of G proteins with GPCRs visualized by two-photon polarization microscopy // 60th Annual meeting of the Biophysical Society, Los Angeles CA, USA (Oral presentation)
2016 Bondar A., Lazar J. Studies of G proteins preassembly with GPCRs with two-photon polarization microscopy // Keystone Symposium G Protein-Coupled Receptors: Structure, Signaling and Drug Discovery, Keystone CO, USA (Oral presentation)
2015 Bondar A. Studies of protein structure and function using polarization microscopy // Annual meeting of Czechoslovak Microscopy Society, Lednice, Czech Republic (Invited talk)
2014 Bondar A., Lazar J. In vivo imaging and quantitation of interactions between Gi/o protein subunits and GPCRs by two-photon polarization microscopy // Keystone Symposium G protein-coupled receptors: structural dynamics and functional implications, Snowbird UT, USA (Oral presentation)
Tutoring of students:
2009-2019 Annual Summer School in Molecular Biophysics and Systems Biology, Nové Hrady, Czech Republic (Project leader: one-month project, 2 students/year)
2021-2022 Bachelor’s students of the joint Biochemistry program of the Johannes Kepler University in Linz and the University of South Bohemia in České Budějovice (2 students)
2022-2023 Bachelor’s student of the Faculty of Science (1 student)
Scientific Interests:
- Mechanisms of GPCR/G protein signal transduction
- Imaging and computational techniques for studies of membrane proteins
- Development of optical sensors for cell signaling processes
Member of:
COST Action ERNEST – Management Committee member, Workgroup 4 Co-Leader.
American Society for Neuroscience
American Society for Biochemistry and Molecular Biology
Biophysical society
Czech Society for Biochemistry and Molecular Biology (FEBS constituent society)
Czechoslovak Microscopy Society (EMS constituent society)
Languages Spoken:
Fluent: Russian (native), English (advanced), Czech (intermediate); other: Belarusian, German
Creative activity
Current schedule
Information
Budweis Single-Molecule Group
Our research group is taking the advantage of living on the border between biology and physics. We are mostly focused on single-molecule research and we are currently looking for motivated students of all degrees!
Right now we are looking for motivated students of all levels (Bc, Mgr, PhD)!
Contact information
This email address is being protected from spambots. You need JavaScript enabled to view it.
This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.
Address: Building C (room 01037) Faculty of Science University of South Bohemia in České Budějovice Branišovská 1760 370 05 České Budějovice
Studies of G protein signaling at the single molecule level
- Getting insights into non-canonical G protein signaling and development of signaling activity sensors
Motivation:
The G protein signaling cascade is a major pathway responsible for cellular communication with the external environment. It is present in all eukaryotes from yeast to humans. G proteins transduce signals from a variety of chemical and physical stimuli including hormones, odorants, neurotransmitters, and light. Up to 50% of all modern prescription drugs target this signaling cascade. However, many aspects of G protein signaling remain unclear. Our goal is to gain insights into non-conventional properties of G protein signaling in mammalian and fungal cells. Particularly, we are interested in precoupling between G proteins and G protein-coupled receptors (GPCRs), dimerization and heteromerization of GPCSs, and development of signaling activity sensors (1-5). In our research we use advanced imaging techniques, including single-molecule imaging, combined with molecular biology, cell biology methods and advanced data processing. We strive to utilize cutting-edge techniques and multidisciplinary approaches to do excellent science.
Publications:
Petelak A., Lambert N.A., Bondar A. 5-HT7 receptor slows down the Gs protein: a single molecule perspective Molecular Biology of the Cell (2023)
A. Bondar, J. Lazar, Optical sensors of heterotrimeric G protein signaling. FEBS J 288, 2570-2584 (2021).
A. Bondar, O. Rybakova, J. Melcr, J. Dohnalek, P. Khoroshyy, O. Tichacek, S. Timr, P. Miclea, A. Sakhi, V. Markova, J. Lazar Quantitative Linear Dichroism Imaging of Molecular Processes in Living Cells Made Simple by Open Software Tools Communications Biology 4, 189 (2021).
A. Bondar, W. Jang, E. Sviridova, N. A. Lambert, Components of the Gs signaling cascade exhibit distinct changes in mobility and membrane domain localization upon beta2-adrenergic receptor activation. Traffic 21, 324-332 (2020).
A. Bondar, J. Lazar, The G protein Gi1 exhibits basal coupling but not preassembly with G protein-coupled receptors. J Biol Chem 292, 9690-9698 (2017).
A. Bondar, J. Lazar, Dissociated GalphaGTP and Gbetagamma protein subunits are the major activated form of heterotrimeric Gi/o proteins. J Biol Chem 289, 1271-1281 (2014).
Branišovská 1645/31a, 370 05 České BudějoviceTel.+420 389 032 191 | This email address is being protected from spambots. You need JavaScript enabled to view it.
Branišovská 1645/31a, 370 05 České BudějoviceTel.+420 389 032 191This email address is being protected from spambots. You need JavaScript enabled to view it.www.jcu.cz
